ORCID

https://orcid.org/0009-0001-0850-8197

Department

Biological Sciences

Year of Study

1

Full-time or Part-time Study

Full-time

Level

Postgraduate

Presentation Type

Poster

Supervisor

Dr Francesca Bottacini

Supervisor

Prof Douwe van Sinderen

Abstract

Bifidobacteria are beneficial commensals of the human gastrointestinal tract and their presence in the gut has been associated with positive health effects on the host. They account for a vast proportion of the infant gut microbiota, when the infant is fed on a milk-based diet, with their number progressively decreasing in adult and elderly. The gut microbiota and associated metabolic activities significantly impact on human health by promoting appropriate development of the infant immune system and contributing to the maintenance of the intestinal homeostasis. In recent years it has become clear that microbial colonization of the gut immediately following birth represents a crucial phase in the development of host metabolism and physiology, in some case promoted by prebiotic substrates such as Human Milk Oligosaccharides (HMOs). For this reason, gut microbiota research has shown particular interest on early colonisers of the infant gut capable of metabolising those prebiotic substrates. In the current study an extensive comparative and functional genomic analysis of 10 novel bifidobacterial isolates have highlighted the presence of genetic loci responsible for HMOs utilisation, production of extracellular structures involved in bacteria-host interaction and production of antimicrobials in the strains, thus showing how comparative genomic approaches can support the phenotypic characterisation of novel bifidobacterial isolates.

Keywords:

bifidobacteria, gut microbiota, HMOs, prebiotic substrates, bacteria-host interaction, antimicrobials, extracellular structures

Start Date

2-11-2023 11:15 AM

End Date

2-11-2023 12:00 PM

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Nov 2nd, 11:15 AM Nov 2nd, 12:00 PM

Genomic diversity and carbohydrate utilisation in human-associated bifidobacterial isolates

Bifidobacteria are beneficial commensals of the human gastrointestinal tract and their presence in the gut has been associated with positive health effects on the host. They account for a vast proportion of the infant gut microbiota, when the infant is fed on a milk-based diet, with their number progressively decreasing in adult and elderly. The gut microbiota and associated metabolic activities significantly impact on human health by promoting appropriate development of the infant immune system and contributing to the maintenance of the intestinal homeostasis. In recent years it has become clear that microbial colonization of the gut immediately following birth represents a crucial phase in the development of host metabolism and physiology, in some case promoted by prebiotic substrates such as Human Milk Oligosaccharides (HMOs). For this reason, gut microbiota research has shown particular interest on early colonisers of the infant gut capable of metabolising those prebiotic substrates. In the current study an extensive comparative and functional genomic analysis of 10 novel bifidobacterial isolates have highlighted the presence of genetic loci responsible for HMOs utilisation, production of extracellular structures involved in bacteria-host interaction and production of antimicrobials in the strains, thus showing how comparative genomic approaches can support the phenotypic characterisation of novel bifidobacterial isolates.