ORCID
0000-0003-4414-7664
Department
Biological Sciences
Year of Study
2
Full-time or Part-time Study
Full-time
Level
Postgraduate
Presentation Type
Poster
Supervisor
Dr. Caitriona Guinane
Supervisor
Dr. Craig Murphy
Supervisor
Dr. Karen Finn
Abstract
Background
Pancreatic cancer (PC) is a prevalent cancer accounting for over 450,000 deaths globally in 2020, and is associated with limited treatment options and high mortality rates. Mounting evidence implicates the human microbiome in tumorigenesis and cancer progression with key players including Helicobacter pylori in gastric cancer and Fusobacterium nucleatum in colorectal cancer and PC. Current research, however, also suggests a potential role for certain microbial residents in oncological treatment and biotherapeutic development. Recent studies have shown a link between cell-free supernatants (CFS) from probiotic strains and cancer cell death, suggesting their potential in targeting the tumour microenvironment. Probiotic genera including Lactobacillus and Bifidobacterium have been highlighted in this regard.
Aims
Few studies have evaluated the effect of probiotic CFS on PC cell lines. The initial aim of this research is to investigate the potential cytotoxicity of various Lactobacillus strains on primary and metastatic PC cell lines.
Future Research plans
Preliminary work has indicated a significant reduction in cancer cell viability when Panc-1 cells were exposed to CFS from selected Lactobacillus strains analysed using MTS assays. Ongoing studies will examine the impact of liquid and freeze-dried CFS produced by Lactobacillus and Bifidobacterial strains on PC in vitro.
Proposed Significance to the Field of Research
Given PC’s limited treatment options and low survival rates, the identification and development of a microbial-based therapeutic candidate would be beneficial in developing novel treatment options for this cancer.
Keywords:
Human microbiome, Pancreatic cancer, Microbial therapies, Probiotics
Start Date
June 2022
End Date
June 2022
Recommended Citation
Doocey, Claire M., "'Investigating the effect of cell-free supernatant of probiotic strains on the viability of pancreatic cancer cell lines'" (2022). ORBioM (Open Research BioSciences Meeting). 12.
https://sword.cit.ie/orbiom/2022/posters/12
Included in
'Investigating the effect of cell-free supernatant of probiotic strains on the viability of pancreatic cancer cell lines'
Background
Pancreatic cancer (PC) is a prevalent cancer accounting for over 450,000 deaths globally in 2020, and is associated with limited treatment options and high mortality rates. Mounting evidence implicates the human microbiome in tumorigenesis and cancer progression with key players including Helicobacter pylori in gastric cancer and Fusobacterium nucleatum in colorectal cancer and PC. Current research, however, also suggests a potential role for certain microbial residents in oncological treatment and biotherapeutic development. Recent studies have shown a link between cell-free supernatants (CFS) from probiotic strains and cancer cell death, suggesting their potential in targeting the tumour microenvironment. Probiotic genera including Lactobacillus and Bifidobacterium have been highlighted in this regard.
Aims
Few studies have evaluated the effect of probiotic CFS on PC cell lines. The initial aim of this research is to investigate the potential cytotoxicity of various Lactobacillus strains on primary and metastatic PC cell lines.
Future Research plans
Preliminary work has indicated a significant reduction in cancer cell viability when Panc-1 cells were exposed to CFS from selected Lactobacillus strains analysed using MTS assays. Ongoing studies will examine the impact of liquid and freeze-dried CFS produced by Lactobacillus and Bifidobacterial strains on PC in vitro.
Proposed Significance to the Field of Research
Given PC’s limited treatment options and low survival rates, the identification and development of a microbial-based therapeutic candidate would be beneficial in developing novel treatment options for this cancer.