Title

The role of the Cronobacter sakazakii ProP C-terminal coiled coil domain in osmotolerance

Document Type

Article

Disciplines

Biology

Publication Details

Gut Pathogens

Abstract

© 2014 Feeney et al.; licensee BioMed Central Ltd. Background: We investigate the role of the C-terminal coiled coil of the secondary proline porter ProP in contributing to Cronobacter sakazakii osmotolerance. Findings: The extended C-terminal domain of ProP1 (encoded by ESA-02131) was spliced onto the truncated C-terminal end of ProP2 (encoded by ESA-01706); creating a chimeric protein (ProPc) which exhibits increased osmotolerance relative to the wild type. Conclusions: It appears that the C-terminal coiled coil domain tunes ProP at low osmolality, whereas ProP transporters lacking the coiled coil domain are more active at a higher osmolality range.

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