The role of the Cronobacter sakazakii ProP C-terminal coiled coil domain in osmotolerance

Authors

Audrey Feeney, Cork Institute of Technology
Christopher D. Johnston, Cork Institute of Technology
Alan Lucid, Cork Institute of Technology
Jim O'Mahony, Department of Biological Sciences, Cork Institute of Technology, Cork, IrelandFollow
Aidan Coffey, Department of Biological Sciences, Cork Institute of Technology, Cork, IrelandFollow
Brigid Lucey, Department of Biological Sciences, Cork Institute of Technology, Cork, IrelandFollow
Roy D. Sleator, Department of Biological Sciences, Cork Institute of Technology, Cork, IrelandFollow

Document Type

Article

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Disciplines

Biology

Publication Details

Gut Pathogens

Abstract

© 2014 Feeney et al.; licensee BioMed Central Ltd. Background: We investigate the role of the C-terminal coiled coil of the secondary proline porter ProP in contributing to Cronobacter sakazakii osmotolerance. Findings: The extended C-terminal domain of ProP1 (encoded by ESA-02131) was spliced onto the truncated C-terminal end of ProP2 (encoded by ESA-01706); creating a chimeric protein (ProPc) which exhibits increased osmotolerance relative to the wild type. Conclusions: It appears that the C-terminal coiled coil domain tunes ProP at low osmolality, whereas ProP transporters lacking the coiled coil domain are more active at a higher osmolality range.

Recommended Citation

Feeney, Audrey; Johnston, Christopher D.; Lucid, Alan; O'Mahony, Jim; Coffey, Aidan; Lucey, Brigid; and Sleator, Roy D., "The role of the Cronobacter sakazakii ProP C-terminal coiled coil domain in osmotolerance" (2014). Department of Biological Sciences Publications [online].
Available at: https://doi.org/10.1186/s13099-014-0046-9