Insertional inactivation of determinants for Mg²⁺ and Co ²⁺ transport as a tool for screening recombinant Lactococcus species clones

Authors

Susan Mills, Moorepark Animal & Grassland Research Centre
Aidan Coffey, Department of Biological Sciences, Cork Institute of Technology, Cork, IrelandFollow
Colin Hill, University College Cork
Gerald F. Fitzgerald, University College Cork
Olivia McAuliffe, Moorepark Animal & Grassland Research Centre
R. Paul Ross, Moorepark Animal & Grassland Research Centre

Document Type

Article

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Disciplines

Biology

Publication Details

Applied and Environmental Microbiology

Abstract

Insertional inactivation of the plasmid-encoded determinants for Mg 2+ and Co2+ transport, orf18/corA, provides a tool for screening recombinant clones in Lactococcus, based on the observation that overexpression of orfl8/corA results in cell growth inhibition on certain concentrations of CoCl2. The lacticin 3147 immunity gene, ltnI, was used to insertionally inactivate orfl8/corA. The resulting clones were capable of growth on concentrations of CoCl2 that were inhibitory to the parent strain. Since only 3 of 17 lactococcal starters naturally harbor cor A, the system has potential as a screen for selecting recombinant lactococcal clones. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Recommended Citation

Mills, Susan; Coffey, Aidan; Hill, Colin; Fitzgerald, Gerald F.; McAuliffe, Olivia; and Ross, R. Paul, "Insertional inactivation of determinants for Mg²⁺ and Co ²⁺ transport as a tool for screening recombinant Lactococcus species clones" (2005). Department of Biological Sciences Publications [online].
Available at: https://doi.org/10.1128/AEM.71.8.4897-4901.2005