Title

Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue

Document Type

Article

Disciplines

Biology

Publication Details

Applied and Environmental Microbiology

Abstract

A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 104 to 108 CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis. Copyright © 2009, American Society for Microbiology. All Rights Reserved.

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