Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue
A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 104 to 108 CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Carroll, James; Douarre, Pierre; Coffey, Aidan; Buckley, Jim; Cashman, Bill; O'Farrell, Kevin; and O'Mahony, Jim, "Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue" (2009). Department of Biological Sciences Publications [online].
Available at: https://doi.org/10.1128/AEM.01203-09