Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue

Authors

James Carroll, Cork Institute of Technology
Pierre Douarre, Cork Institute of Technology
Aidan Coffey, Department of Biological Sciences, Cork Institute of Technology, Cork, IrelandFollow
Jim Buckley, Cork County Council
Bill Cashman, Cork County Council
Kevin O'Farrell, Cork County Council
Jim O'Mahony, Department of Biological Sciences, Cork Institute of Technology, Cork, IrelandFollow

Document Type

Article

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Disciplines

Biology

Publication Details

Applied and Environmental Microbiology

Abstract

A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 104 to 108 CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis. Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Recommended Citation

Carroll, James; Douarre, Pierre; Coffey, Aidan; Buckley, Jim; Cashman, Bill; O'Farrell, Kevin; and O'Mahony, Jim, "Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue" (2009). Department of Biological Sciences Publications [online].
Available at: https://doi.org/10.1128/AEM.01203-09