The truncated phage lysin CHAP_k eliminates staphylococcus aureus in the nares of mice

Authors

Mark Fenton, Cork Institute of Technology
Pat G. Casey, APC Microbiome Ireland
Colin Hill, APC Microbiome Ireland
Cormac G.M. Gahan, APC Microbiome Ireland
R. Paul Ross, Moorepark Food Research Centre
Olivia Mcauliffe, Moorepark Food Research Centre
Jim O'Mahony, Cork Institute of Technology
Fiona Maher, Cork Institute of Technology
Aidan Coffey, Cork Institute of Technology

Document Type

Article

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Disciplines

Biology

Publication Details

Bioengineered Bugs

Abstract

The endolysin LysK derived from staphylococcal phage K has previously been shown to have two enzymatic domains, one of which is an N-acetylmuramoyl-L-alanine amidase and the other a cysteine/histidine dependant amidohydrolase/ peptidase designated CHAPk. The latter, when cloned as a single-domain truncated enzyme, is conveniently overexpressed in a highly-soluble form. This enzyme was shown to be highly active in vitro against live cell suspensions of S. aureus. In the current study, the IVIS imaging system was used to demonstrate the effective elimination of a lux labeled S. aureus from the nares of BALB/c mice. © 2010 Landes Bioscience.

Recommended Citation

Fenton, Mark; Casey, Pat G.; Hill, Colin; Gahan, Cormac G.M.; Ross, R. Paul; Mcauliffe, Olivia; O'Mahony, Jim; Maher, Fiona; and Coffey, Aidan, "The truncated phage lysin CHAP_k eliminates staphylococcus aureus in the nares of mice" (2010). Department of Biological Sciences Publications [online].
Available at: https://doi.org/10.4161/bbug.1.6.13422