ORCID

https://orcid.org/0000-0002-0872-202X

Document Type

Preprint

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Disciplines

1.4 CHEMICAL SCIENCES; 1.6 BIOLOGICAL SCIENCES; Microbiology

Disciplines

Biology | Chemicals and Drugs | Diseases | Medicine and Health Sciences | Microbiology | Virus Diseases

Publication Details

This is a pre-print article. It has not gone through the peer-review process and is being made available with urgency to help combat the global COVID-19 Crisis.

Abstract

The COVID-19 pandemic has resulted in increased need for diagnostic testing using reverse transcriptase real-time PCR (RT-PCR). An exponential increase in demand has resulted in a shortage of numerous reagents in particular those associated with the lysis buffer required to extract the viral RNA. Herein, we describe a rapid collective effort by hospital laboratory scientists, academic researchers and the biopharma industry to generate a validated lysis buffer. We have formulated a 4M Guanidinium thiocyanate (GITC)/ Triton X-100 Lysis buffer which provides comparable results with the recommended reagents. This buffer will ease the burden on hospital labs in their heroic efforts diagnose a large population of patients.

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