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Biochemistry, Biophysics, and Structural Biology | Biology | Biotechnology | Cell and Developmental Biology | Food Science | Genetics and Genomics | Medicine and Health Sciences | Microbiology | Nutrition | Other Medicine and Health Sciences | Pharmacology, Toxicology and Environmental Health | Physiology | Public Health
3. MEDICAL AND HEALTH SCIENCES; 3.3 HEALTH SCIENCES; Integrative and complementary medicine; Nutrition, Dietetics; Public and environmental health; 3.4 HEALTH BIOTECHNOLOGY; Technologies involving identifying the functioning of DNA,; proteins and enzymes and how they influence
The human gut microbiome consists of at least 3 million non-redundant genes, 150 times that of the core human genome. Herein, we report the identification and characterisation of a novel stress tolerance gene from the human gut metagenome. The locus, assigned brpA, encodes a membrane protein with homology to a brp/blh-family β-carotene monooxygenase. Cloning and heterologous expression of brpA in Escherichia coli confers a significant salt tolerance phenotype. Furthermore, when cultured in the presence of exogenous β-carotene, cell pellets adopt a red/orange pigmentation indicating the incorporation of carotenoids in the cell membrane.
Culligan EP, Sleator RD, Marchesi JR, Hill C (2014) Metagenomic Identification of a Novel Salt Tolerance Gene from the Human Gut Microbiome Which Encodes a Membrane Protein with Homology to a brp/blh-Family β-Carotene 15,15′-Monooxygenase. PLoS ONE 9(7): e103318. https://doi.org/10.1371/journal.pone.0103318
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