Document Type

Article

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Disciplines

Bacteriology | Biochemistry | Biology | Biotechnology | Food Microbiology | Genetics and Genomics | Genomics | Microbial Physiology | Microbiology | Organismal Biological Physiology | Pathogenic Microbiology

CIT Disciplines

1.6 BIOLOGICAL SCIENCES; Microbiology; Biochemistry and molecular biology

Publication Details

Bioengineered

Abstract

Cronobacter sakazakii is a neonatal pathogen responsible for up to 80% of fatalities in infected infants. Low birth weight infants and neonates infected with C. sakazakii suffer necrotizing enterocolitis, bacteraemia and meningitis. The mode of transmission most often associated with infection is powdered infant formula (PIF) which, with an aw of ∼0.2, is too low to allow most microorganisms to persist. Survival of C. sakazakii in environments subject to extreme hyperosmotic stress has previously been attributed to the uptake of compatible solutes including proline and betaine. Herein, we report the construction and screening of a C. sakazakii genome bank and the identification of ProP (ESA_02131) as a carnitine uptake system.

Table 1. Bacterial strains and plasmids.csv (1 kB)
Bacterial strains and plasmids

Table 2. Primers.csv (1 kB)
Primers

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