Document Type

Article

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Disciplines

Biochemistry, Biophysics, and Structural Biology | Biotechnology | Food Biotechnology | Food Microbiology | Food Science

CIT Disciplines

1.6 BIOLOGICAL SCIENCES; Microbiology; Virology; Biochemistry and molecular biology; 1.7 OTHER NATURAL SCIENCES

Publication Details

Bioengineered

Abstract

Betaine uptake in Listeria monocytogenes is mediated by three independent transport systems, the simplest of which in genetic terms is the secondary transporter BetL. Using a random mutagenesis approach, based on the E. coli XL1 Red mutator strain, we identified a single point mutation in a putative promoter region upstream of the BetL coding region which leads to a significant increase in betL transcript levels under osmo- and chill-stress conditions and a concomitant increase in stress tolerance. Furthermore, the mutation appears to counter the heretofore unreported “twisted” cell morphology observed for L. monocytogenes grown at elevated osmolarities in tryptone soy broth.

Hoffman 2013 et al T1-10.4161_bioe.24094.csv (1 kB)
Table 1. Bacterial strains and plasmids

Hoffman et al 2013 T2-10.4161_bioe.24094.csv (1 kB)
Table 2. PCR primers used in this study

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