Primer,Sequence (5′-3′)
betLFPstΙ,CATCTGCAGGCTTTCTCCCCCTTTTTCCTC
betLRXbaΙ,CATTCTAGAGCTCTATTCCAATTACCGCCATTTC
XbaIKO,TAAGCGCCACTCTAGACC
EcoRIKO,GCACGAATTCACCAAGTA
U141,TTGCTCTTCCAATGTTAG
L142,GAGTGCTTAATGCGTTAG