Date of Award


Document Type

Master Thesis

Degree Name

Masters of Science (Research)


Biological Sciences

First Advisor

Dr. John O'Mullane


Cardiac troponin I (cTnl) has been reported to be a highly specific marker for myocardial injury. In patients suffering from acute coronary syndromes, a sensitive serum marker which is closely related to the extent of myocardial damage which provides prognostic data and which can be measured rapidly is desirable. In this thesis a “sandwich” enzyme immunoassay was developed to measure cTnI. The developed assay was reproducible with the inter assay precision ranging from 4.8-14% and the intra assay precision ranging from 3.1-9.5%. The limit of detection of the assay was calculated as 0.385 ng/ml. The upper reference range (non-parametric 95% interval) was established as 28.2 ng/ml by measuring the cTnl concentration in sera of 128 blood donor samples. A correlation study was performed between the developed assay and another established method i.e. the Troponin I Pasteur assay using samples from “normal” and query acute myocardial infarction (AMI) patients. Despite achieving poor correlation, with modifications the developed assay could prove invaluable in the detection of AMI.


A thesis presented to the National Council for Educational Awards for the Degree of Master of Science.

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