Date of Award
Masters of Science (Research)
Dr. John O'Mullane
Cardiac troponin I (cTnl) has been reported to be a highly specific marker for myocardial injury. In patients suffering from acute coronary syndromes, a sensitive serum marker which is closely related to the extent of myocardial damage which provides prognostic data and which can be measured rapidly is desirable. In this thesis a “sandwich” enzyme immunoassay was developed to measure cTnI. The developed assay was reproducible with the inter assay precision ranging from 4.8-14% and the intra assay precision ranging from 3.1-9.5%. The limit of detection of the assay was calculated as 0.385 ng/ml. The upper reference range (non-parametric 95% interval) was established as 28.2 ng/ml by measuring the cTnl concentration in sera of 128 blood donor samples. A correlation study was performed between the developed assay and another established method i.e. the Troponin I Pasteur assay using samples from “normal” and query acute myocardial infarction (AMI) patients. Despite achieving poor correlation, with modifications the developed assay could prove invaluable in the detection of AMI.
Blake, William G., "Development and Validation of an Improved Ultrasensitive Assay for Cardiac Troponin I (cTnI)" (1998). Theses [online].
Available at: https://sword.cit.ie/allthe/348